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影响因子：3.395

DOI码：10.1016/j.cj.2020.02.005

发表刊物：The Crop Journal

关键字：AAP gene family， Gain protein content，grain quality

摘要：High grain protein content (GPC) reduces rice eating and cooking quality (ECQ). We generated OsAAP6 and OsAAP10 knockout mutants in three high-yielding japonica varieties and one japonica line using the CRISPR/Cas9 system. Mutation efficiency varied with genetic background in the T0 generation, and GPC in the T1 generation decreased significantly, owing mainly to a reduction in glutelin content. Amylose content was down regulated significantly in some Osaap6 and all Osaap10 mutants. The increased taste value of these mutants was supported by Rapid Visco Analysis (RVA) profiles, which showed higher peak viscosity and breakdown viscosity and lower setback viscosity than the wild type. There were no significant deficiencies in agronomic traits of the mutants. Targeted mutagenesis of OsAAP6 and OsAAP10, especially OsAAP10, using the CRISPR/Cas9 system can rapidly reduce GPC and improve ECQ of rice, providing a new strategy for the breeding cultivars with desired ECQ.

论文类型：SCI

学科门类：农学

一级学科：作物学

文献类型：SCI

卷号：8

期号：3

页面范围：457-464

ISSN号：22145141

是否译文：否

发表时间：2020-03-25

收录刊物：SCI