Targeted mutagenesis of amino acid transporter genes for rice quality improvement using the CRISPR/Cas9 system
Date:2021-08-23 clicks:
Impact Factor:3.395
DOI number:10.1016/j.cj.2020.02.005
Journal:The Crop Journal
Key Words:AAP gene family， Gain protein content，grain quality
Abstract:High grain protein content (GPC) reduces rice eating and cooking quality (ECQ). We generated OsAAP6 and OsAAP10 knockout mutants in three high-yielding japonica varieties and one japonica line using the CRISPR/Cas9 system. Mutation efficiency varied with genetic background in the T0 generation, and GPC in the T1 generation decreased significantly, owing mainly to a reduction in glutelin content. Amylose content was down regulated significantly in some Osaap6 and all Osaap10 mutants. The increased taste value of these mutants was supported by Rapid Visco Analysis (RVA) profiles, which showed higher peak viscosity and breakdown viscosity and lower setback viscosity than the wild type. There were no significant deficiencies in agronomic traits of the mutants. Targeted mutagenesis of OsAAP6 and OsAAP10, especially OsAAP10, using the CRISPR/Cas9 system can rapidly reduce GPC and improve ECQ of rice, providing a new strategy for the breeding cultivars with desired ECQ.
Indexed by:SCI
Discipline:Agricultural science
First-Level Discipline:Crop Science
Document Type:SCI
Volume:8
Issue:3
Page Number:457-464
ISSN No.:22145141
Translation or Not:no
Date of Publication:2020-03-25
Included Journals:SCI
